[ccp4bb] air bubbles in the Bio-Rad Duoflow system

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Parameter MAXSAVE exceeded
From: Jayashankar s {- dot -} jayashankar {- at -} GMAIL {- dot -} COM
Date: 2008-07-04

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From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2008-07-04

Subject: air bubbles in the Bio-Rad Duoflow system
From: Ailong Ke ak425 {- at -} CORNELL {- dot -} EDU
Date: 2008-07-04

Hello All,

I own a Bio-Rad Duoflow system for almost a year. The machine lived
up to some of the recommendations I saw on this board. However, there
is this annoying problem with air bubbles entering columns and I
cannot figure out the source. The system would be free of any air
bubbles in the beginning, and performs fine when water is loaded
using needle/syringe into the 5ml superloop and then injected into
the column. When protein samples are injected the same way, a lot of
air bubbles would appear and get trapped inside the ion exchange
column, leaving yellowish marks on the Uno columns and eventually
reducing their performance. I've been a FLC/AKTA user for about ten
years and have never seen such problems. I doubt it is due to a
faulty injection valve because we recently had it replaced for other
reasons. We did move a backpressure generator (a little black piece)
from post-column position to before-column, otherwise we cannot run
sizing columns in a reasonable flow rate.

I'd like to hear if you have similar experiences and how you fixed
the problem. Thanks a lot!

Ailong

--

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Parameter MAXSAVE exceeded
From: Jayashankar s {- dot -} jayashankar {- at -} GMAIL {- dot -} COM
Date: 2008-07-04

Next message:
Subject: Small lysozyme crystals protocol
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2008-07-04