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Re: [ccp4bb] phosphorylation of protein is troublesome or not for crystallization |
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CCP4bb navigationCCP4bb <-- 2007 <-- April 2007 <-- 17 April 2007Subject: Re: phosphorylation of protein is troublesome or not for crystallization From: Kurt Hoffmann hoffmann {- at -} MOLBIOTECH {- dot -} RWTH-AACHEN {- dot -} DE Date: 2007-04-17 please find an example of a protein, which showed multiple phosphorylations. Proteins were separated using a strong anion exchange chromatography (SAX, smaller elution gradient). Only once the single phosphorylated forms were isolated we obtained nice crystals. I think no special crystallization techniques need to be employed, but protein should be homogenous. I used our mass-spec (ESI-Q-TOf-II) to characterize the protein and its different elutions from the SAX (slide II). Regards, Kurt Hoffmann RWTH-Aachen _____ From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Rongjin Guan Sent: Montag, 16. April 2007 23:59 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] phosphorylation of protein is troublesome or not for crystallization Dear all, Below is a question my friend asked me, but I have never worked on phosphorylated proteins. Has anyone worked on crystallizing phosphorylated proteins and can you comment on it? Thanks Rongjin Guan ---------------------------------------------------------------------- I would like to ask how feasible it is to crystalize protein containing post-translational modification, such as phosphorylation. To my limited knowledge, I think the heterogenicity of modified and unmodified proteins causes the major obstacle in the crystal structure. Is there any specific method to crystalized modified proteins (like phosphorylated proteins ) these days? CCP4bb navigationCCP4bb <-- 2007 <-- April 2007 <-- 17 April 2007 |
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