Re: [ccp4bb] phosphorylation of protein is troublesome or not for crystallization

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: phosphorylation of protein is troublesome or not for crystallization
From: Rongjin Guan rjguan {- at -} GMAIL {- dot -} COM
Date: 2007-04-16

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From: David Briggs bassophile {- at -} GMAIL {- dot -} COM
Date: 2007-04-17

Subject: Re: phosphorylation of protein is troublesome or not for crystallization
From: Kurt Hoffmann hoffmann {- at -} MOLBIOTECH {- dot -} RWTH-AACHEN {- dot -} DE
Date: 2007-04-17

Dear Guan,

please find an example of a protein, which showed multiple phosphorylations.

Proteins were separated using a strong anion exchange chromatography (SAX,
smaller elution gradient).

Only once the single phosphorylated forms were isolated we obtained nice
crystals.

I think no special crystallization techniques need to be employed, but
protein should be homogenous.

I used our mass-spec (ESI-Q-TOf-II) to characterize the protein and its
different elutions from the SAX (slide II).

Regards,

Kurt Hoffmann

RWTH-Aachen

_____

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Rongjin Guan
Sent: Montag, 16. April 2007 23:59
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] phosphorylation of protein is troublesome or not for
crystallization

Dear all,

Below is a question my friend asked me, but I have never worked on
phosphorylated proteins.

Has anyone worked on crystallizing phosphorylated proteins and can you
comment on it?

Thanks

Rongjin Guan

----------------------------------------------------------------------

I would like to ask how feasible it is to crystalize protein containing
post-translational modification, such as phosphorylation. To my limited
knowledge, I think the heterogenicity of modified and unmodified proteins
causes the major obstacle in the crystal structure. Is there any specific
method to crystalized modified proteins (like phosphorylated proteins )
these days?

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: phosphorylation of protein is troublesome or not for crystallization
From: Rongjin Guan rjguan {- at -} GMAIL {- dot -} COM
Date: 2007-04-16

Next message:
Subject: Differentiating bound Mn & Ca - Summary
From: David Briggs bassophile {- at -} GMAIL {- dot -} COM
Date: 2007-04-17