[ccp4bb] Small lysozyme crystals protocol

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: air bubbles in the Bio-Rad Duoflow system
From: Ailong Ke ak425 {- at -} CORNELL {- dot -} EDU
Date: 2008-07-04

Next message:
Subject: Re: air bubbles in the Bio-Rad Duoflow system
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-07-04

Subject: Small lysozyme crystals protocol
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2008-07-04

Dear Crystallogrphers,

does anybody here know a protocol to get consistently well-diffracting but
smaller, ~50um, cryoprotect(ed/able) lysozyme crystals?

Thanks,

Jacob Keller

*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-keller2@northwestern.edu
*******************************************

----- Original Message -----
From: "Ailong Ke"
To:
Sent: Thursday, July 03, 2008 4:13 PM
Subject: [ccp4bb] air bubbles in the Bio-Rad Duoflow system

> Hello All,
>
> I own a Bio-Rad Duoflow system for almost a year. The machine lived up to
> some of the recommendations I saw on this board. However, there is this
> annoying problem with air bubbles entering columns and I cannot figure out
> the source. The system would be free of any air bubbles in the beginning,
> and performs fine when water is loaded using needle/syringe into the 5ml
> superloop and then injected into the column. When protein samples are
> injected the same way, a lot of air bubbles would appear and get trapped
> inside the ion exchange column, leaving yellowish marks on the Uno columns
> and eventually reducing their performance. I've been a FLC/AKTA user for
> about ten years and have never seen such problems. I doubt it is due to a
> faulty injection valve because we recently had it replaced for other
> reasons. We did move a backpressure generator (a little black piece) from
> post-column position to before-column, otherwise we cannot run sizing
> columns in a reasonable flow rate.
>
> I'd like to hear if you have similar experiences and how you fixed the
> problem. Thanks a lot!
>
> Ailong
>
> --
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: air bubbles in the Bio-Rad Duoflow system
From: Ailong Ke ak425 {- at -} CORNELL {- dot -} EDU
Date: 2008-07-04

Next message:
Subject: Re: air bubbles in the Bio-Rad Duoflow system
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-07-04