[ccp4bb] small lysozyme crystals

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Small lysozyme crystals protocol
From: "Mathews, Irimpan" iimathew {- at -} SLAC {- dot -} STANFORD {- dot -} EDU
Date: 2008-07-04

Next message:
Subject: job opening at AstraZeneca, Sweden
From: "Käck,_Helena" helena {- dot -} kack {- at -} ASTRAZENECA {- dot -} COM
Date: 2008-07-04

Subject: small lysozyme crystals
From: McEwan Paul Paul {- dot -} Mcewan {- at -} NOTTINGHAM {- dot -} AC {- dot -} UK
Date: 2008-07-04

Use the 15 minute protocol from the Hampton Research catalogue/website. They're normally quite small and easy to cryoprtect with a little glycerol. Size can also be varied by playing with the salt concentration (slightly higher [NaCl] giving more but smaller xtals)

Paul..

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Dr. Paul A. McEwan

Protein Crystallography Group

Office C58

Centre for Biomolecular Science

University of Nottingham

Nottingham

NG7 2RD

Tel: 0115 8232018

http://www.nottingham.ac.uk/pharmacy/research/medicinal-chemistry-structural-biology/structbio.php
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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Small lysozyme crystals protocol
From: "Mathews, Irimpan" iimathew {- at -} SLAC {- dot -} STANFORD {- dot -} EDU
Date: 2008-07-04

Next message:
Subject: job opening at AstraZeneca, Sweden
From: "Käck,_Helena" helena {- dot -} kack {- at -} ASTRAZENECA {- dot -} COM
Date: 2008-07-04