Re: [ccp4bb] Small lysozyme crystals protocol

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Refmac unrestrained refinement on ligands only
From: Ian Tickle I {- dot -} Tickle {- at -} ASTEX-THERAPEUTICS {- dot -} COM
Date: 2008-07-04

Next message:
Subject: Re: Refmac unrestrained refinement on ligands only
From: "George M {- dot -} Sheldrick" gsheldr {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2008-07-04

Subject: Re: Small lysozyme crystals protocol
From: "David J {- dot -} Schuller" djs63 {- at -} CORNELL {- dot -} EDU
Date: 2008-07-04

Rigaku has a page of common crystallization recipes:
http://www.rigaku.com/protein/crystallization.html

Try the "Lysozyme crystallization procedure for low temp use" recipe. It
contains ethylene glycol so the crystals are cryo-ready. To get a large
number of smaller crystals, push the concentration of lysozyme solution
up to 100 - 120 mg/ml.

Cheers,

-
=======================================================================
With the single exception of Cornell, there is not a college in the
United States where truth has ever been a welcome guest - R.G. Ingersoll
=======================================================================
David J. Schuller
modern man in a post-modern world
MacCHESS, Cornell University
schuller@cornell.edu

On Thu, 2008-07-03 at 18:08 -0500, Jacob Keller wrote:
> Dear Crystallogrphers,
>
> does anybody here know a protocol to get consistently well-diffracting but
> smaller, ~50um, cryoprotect(ed/able) lysozyme crystals?
>
> Thanks,
>
> Jacob Keller
>
> *******************************************
> Jacob Pearson Keller
> Northwestern University
> Medical Scientist Training Program
> Dallos Laboratory
> F. Searle 1-240
> 2240 Campus Drive
> Evanston IL 60208
> lab: 847.491.2438
> cel: 773.608.9185
> email: j-keller2@northwestern.edu
> *******************************************
>
> ----- Original Message -----
> From: "Ailong Ke"
> To:
> Sent: Thursday, July 03, 2008 4:13 PM
> Subject: [ccp4bb] air bubbles in the Bio-Rad Duoflow system
>
>
> > Hello All,
> >
> > I own a Bio-Rad Duoflow system for almost a year. The machine lived up to
> > some of the recommendations I saw on this board. However, there is this
> > annoying problem with air bubbles entering columns and I cannot figure out
> > the source. The system would be free of any air bubbles in the beginning,
> > and performs fine when water is loaded using needle/syringe into the 5ml
> > superloop and then injected into the column. When protein samples are
> > injected the same way, a lot of air bubbles would appear and get trapped
> > inside the ion exchange column, leaving yellowish marks on the Uno columns
> > and eventually reducing their performance. I've been a FLC/AKTA user for
> > about ten years and have never seen such problems. I doubt it is due to a
> > faulty injection valve because we recently had it replaced for other
> > reasons. We did move a backpressure generator (a little black piece) from
> > post-column position to before-column, otherwise we cannot run sizing
> > columns in a reasonable flow rate.
> >
> > I'd like to hear if you have similar experiences and how you fixed the
> > problem. Thanks a lot!
> >
> > Ailong
> >
> > --
> >

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Refmac unrestrained refinement on ligands only
From: Ian Tickle I {- dot -} Tickle {- at -} ASTEX-THERAPEUTICS {- dot -} COM
Date: 2008-07-04