[ccp4bb] more on thermofluor

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Refmac unrestrained refinement on ligands only
From: "George M {- dot -} Sheldrick" gsheldr {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2008-07-04

Next message:
Subject: Re: Refmac unrestrained refinement on ligands only
From: Ian Tickle I {- dot -} Tickle {- at -} ASTEX-THERAPEUTICS {- dot -} COM
Date: 2008-07-04

Subject: more on thermofluor
From: Andreas_Förster docandreas {- at -} GMAIL {- dot -} COM
Date: 2008-07-04

Hey there,

we've been demoing real-time PCR machines for use in differential
scanning fluorimetry. Stratagene's Mx3005p has been mentioned before on
this list. It seems to do the job well, and there is great software
support from Frank Niesen at Oxford.

However, we've really come to like Eppendorf's Mastercycler ep realplex
4 because of exceptional results with the proteins we essayed.
Scientifically rigorous superstition requires that we get this
instrument. I was wondering if anyone has any real-life (and not just
real-time) experience with it. In particular, I was wondering how
people set the temperature gradient, how they analyze the data, and how
reliable the instrument is.

Thanks.

Andreas

By the way, the rep told us today that a software particular to protein
melting curve analysis was forthcoming.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Next message:
Subject: Re: Refmac unrestrained refinement on ligands only
From: Ian Tickle I {- dot -} Tickle {- at -} ASTEX-THERAPEUTICS {- dot -} COM
Date: 2008-07-04