- Protein crystallography
Main steps: - Protein purification - Crystallisation
Special: - Programs for crystallography - X-ray detectors
Basic tutorials: - Chemistry - Protein - Peptide - Amino Acids
Xtal community: - CCP4BB
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Subject: From: Daniel Gutmann daniel {- dot -} gutmann {- at -} IMPERIAL {- dot -} AC {- dot -} UK Date: 2007-04-17
Hey there,
I have crystallised a protein in 30% MPD and it it gives nice native data. However, it seems impossible to get heavy metal derivatives and I read that MPD does chelate heavy metals. Did any of you make similar experiences with xtals in MPD conditions? If so did you find a good solution (introduction of selmet makes the protein ustable) to make heavy metal derivatives?
Best Regards
Daniel
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