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Protein crystallography Programs for crystallography X-ray detectors
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Protein Peptide

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From: Daniel Gutmann daniel {- dot -} gutmann {- at -} IMPERIAL {- dot -} AC {- dot -} UK
Date: 2007-04-17

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From: "Mathews, Irimpan" iimathew {- at -} SLAC {- dot -} STANFORD {- dot -} EDU
Date: 2007-04-17

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From: HKim {- at -} DECODE {- dot -} COM HKim {- at -} DECODE {- dot -} COM
Date: 2007-04-17

Hi, Daniel,

Have you tried Br? Do a search on Zbyszek Dauter and halide phasing. Good
luck,

Hidong

Daniel Gutmann
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04/17/2007 10:03 AM
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Hey there,

I have crystallised a protein in 30% MPD and it it gives nice native data.
However, it seems impossible to get heavy metal derivatives and I read
that MPD does chelate heavy metals. Did any of you make similar
experiences with xtals in MPD conditions? If so did you find a good
solution (introduction of selmet makes the protein ustable) to make heavy
metal derivatives?

Best Regards

Daniel

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CCP4bb <-- 2007 <-- April 2007 <-- 17 April 2007

Previous message:
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From: Daniel Gutmann daniel {- dot -} gutmann {- at -} IMPERIAL {- dot -} AC {- dot -} UK
Date: 2007-04-17

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From: "Mathews, Irimpan" iimathew {- at -} SLAC {- dot -} STANFORD {- dot -} EDU
Date: 2007-04-17