[ccp4bb]

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject:
From: Daniel Gutmann daniel {- dot -} gutmann {- at -} IMPERIAL {- dot -} AC {- dot -} UK
Date: 2007-04-17

Next message:
Subject:
From: "Mathews, Irimpan" iimathew {- at -} SLAC {- dot -} STANFORD {- dot -} EDU
Date: 2007-04-17

Subject:
From: HKim {- at -} DECODE {- dot -} COM HKim {- at -} DECODE {- dot -} COM
Date: 2007-04-17

Hi, Daniel,

Have you tried Br? Do a search on Zbyszek Dauter and halide phasing. Good
luck,

Hidong

Daniel Gutmann
Sent by: CCP4 bulletin board
04/17/2007 10:03 AM
Please respond to
Daniel Gutmann

To
CCP4BB@JISCMAIL.AC.UK
cc

Subject
[ccp4bb]

Hey there,

I have crystallised a protein in 30% MPD and it it gives nice native data.
However, it seems impossible to get heavy metal derivatives and I read
that MPD does chelate heavy metals. Did any of you make similar
experiences with xtals in MPD conditions? If so did you find a good
solution (introduction of selmet makes the protein ustable) to make heavy
metal derivatives?

Best Regards

Daniel

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject:
From: Daniel Gutmann daniel {- dot -} gutmann {- at -} IMPERIAL {- dot -} AC {- dot -} UK
Date: 2007-04-17

Next message:
Subject:
From: "Mathews, Irimpan" iimathew {- at -} SLAC {- dot -} STANFORD {- dot -} EDU
Date: 2007-04-17