[ccp4bb]

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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From: HKim {- at -} DECODE {- dot -} COM HKim {- at -} DECODE {- dot -} COM
Date: 2007-04-17

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From: "Das, Debanu" debanu {- at -} SLAC {- dot -} STANFORD {- dot -} EDU
Date: 2007-04-17

Subject:
From: "Mathews, Irimpan" iimathew {- at -} SLAC {- dot -} STANFORD {- dot -} EDU
Date: 2007-04-17

Hi Daniel,

1. You may try Xenon or Kr pressure cell. We have a simple version of these cells at SSRL (see the link below). Rigaku also has slightly more complex version. Let me know if you want to try this and you don't have access to a pressure cell.

http://smb.slac.stanford.edu/public/facilities/hardware/pressurecell/ltpc.html

2. You could try NaBr soaks to make Br derivatives for MAD.

3. There are also some Gd small molecules complexes which may do the trick. See Acta Cryst. D59, 118 (2003).

kind regards,
Mathews

________________________________

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Daniel Gutmann
Sent: Tuesday, April 17, 2007 10:03 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb]

Hey there,

I have crystallised a protein in 30% MPD and it it gives nice native data. However, it seems impossible to get heavy metal derivatives and I read that MPD does chelate heavy metals. Did any of you make similar experiences with xtals in MPD conditions? If so did you find a good solution (introduction of selmet makes the protein ustable) to make heavy metal derivatives?

Best Regards

Daniel

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject:
From: HKim {- at -} DECODE {- dot -} COM HKim {- at -} DECODE {- dot -} COM
Date: 2007-04-17

Next message:
Subject:
From: "Das, Debanu" debanu {- at -} SLAC {- dot -} STANFORD {- dot -} EDU
Date: 2007-04-17