[ccp4bb]

Protein crystallography Programs for crystallography X-ray detectors
Basic tutorials:
Protein Peptide

CCP4bb navigation

CCP4bb <-- 2007 <-- April 2007 <-- 17 April 2007

Previous message:
Subject:
From: "Das, Debanu" debanu {- at -} SLAC {- dot -} STANFORD {- dot -} EDU
Date: 2007-04-17

Next message:
Subject:
From: Kornelius Zeth kornelius {- dot -} zeth {- at -} TUEBINGEN {- dot -} MPG {- dot -} DE
Date: 2007-04-17

Subject:
From: artem {- at -} XTALS {- dot -} ORG artem {- at -} XTALS {- dot -} ORG
Date: 2007-04-17

Hi,

Without knowing exactly what you did, it's hard to guess however here are
a few suggestions, in addition to the ideas already given by others:

a. try iodination. Simply place an iodine crystal near the drop (glue it
to the cover slip using a tiny bit of grease) and let the vapors penetrate
the drop gradually. Also you can add 0.1 mM of KI to the solution then add
a speck of iodine - the KI will slowly transition the iodine via the KI3
complex to the protein. You have to experiment with timing - what worked
for me was 1 day exposure. The crystals may or may not turn yellow -
depends on your protein and on conditions.

b. I am surprised that mercury derivatives did not work. Have you tried
increasing amounts of ethyl mercury phosphate?

c. Have you tried my favorite platinum derivative - KPt(NO2)3? You can add
a LOT of this stuff - it's very mild on the protein.

Artem
>
>
>
> Hey there,
>
> I have crystallised a protein in 30% MPD and it it gives nice native
> data. However, it seems impossible to get heavy metal derivatives and
> I read that MPD does chelate heavy metals. Did any of you make
> similar experiences with xtals in MPD conditions? If so did you find
> a good solution (introduction of selmet makes the protein ustable)
> to make heavy metal derivatives?
>
> Best Regards
>
> Daniel

CCP4bb navigation

CCP4bb <-- 2007 <-- April 2007 <-- 17 April 2007

Previous message:
Subject:
From: "Das, Debanu" debanu {- at -} SLAC {- dot -} STANFORD {- dot -} EDU
Date: 2007-04-17

Next message:
Subject:
From: Kornelius Zeth kornelius {- dot -} zeth {- at -} TUEBINGEN {- dot -} MPG {- dot -} DE
Date: 2007-04-17