[ccp4bb] Cryo with succinate

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: weak protein complexes
From: amit sharma 3112amit {- at -} GMAIL {- dot -} COM
Date: 2008-07-22

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Subject: Re: Cryo with succinate
From: "Van Den Berg, Bert" Lambertus {- dot -} VandenBerg {- at -} UMASSMED {- dot -} EDU
Date: 2008-07-22

Subject: Cryo with succinate
From: Tjaard Pijning t {- dot -} pijning {- at -} RUG {- dot -} NL
Date: 2008-07-22

I have crystals grown from 1 M sodium succinate pH 7 and have been searching for suitable cryo conditions.
Is succinate in itself already a cryoprotectant (maybe at somewhat higher concentration) or should I
add e.g. glycerol ? Any experience or ideas are welcome.

Thanks in advance,

Ing. Tjaard Pijning

Protein Crystallography Group
University of Groningen
Nijenborgh 4
9747 AG Groningen
The Netherlands
Tel. (31)(0)50 363 4385

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: weak protein complexes
From: amit sharma 3112amit {- at -} GMAIL {- dot -} COM
Date: 2008-07-22

Next message:
Subject: Re: Cryo with succinate
From: "Van Den Berg, Bert" Lambertus {- dot -} VandenBerg {- at -} UMASSMED {- dot -} EDU
Date: 2008-07-22