Re: [ccp4bb] offtopic-"colorful tag"

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

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   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: offtopic-"colorful tag"
From: Chun Luo chun {- at -} ACCELAGEN {- dot -} COM
Date: 2008-07-26

Those colorful tags may be too big for crystallization purpose. You can try
GFP or heme based tags. A few companies are promoting heme based red tags.
Red color is not very intense. You'd be better off with UV or GFP. There are
small molecule tags that links to Cys. But you'll spend a fortune to get
enough to see. They are Ni-NTA linked dyes as well.

If your protein expresses well, you can see it while you growing the
culture.

Chun

Chun Luo, Ph.D.
The Protein Expert
Accelagen, Inc.
11585 Sorrento Valley Road, Suite 107
San Diego, CA 92121
TeL: 858-350-8085 ext 111
Fax: 858-350-8001
chun@accelagen.com
www.accelagen.com

_____

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
deliang
Sent: Friday, July 25, 2008 4:26 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] offtopic-"colorful tag"

Hi all,

I am curious whether there is an expression system in which the target
protein will be expressed with a tag, which has a strong extinction
coefficient in visible wavelength range? That will dramatically simplify my
protein purification and crystallization.

Thanks for your suggestion.

Deliang

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Beamline Stability Issues
From: Lucas Sanfelici lsanfelici {- at -} LNLS {- dot -} BR
Date: 2008-07-26

Next message:
Subject: Meaning of sigma level of electron density map?
From: P K greatbiologist {- at -} GMAIL {- dot -} COM
Date: 2008-07-26