[ccp4bb] estimate protein in Incl bod

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: off-topic: protein crystallography in organic chemistry lab
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-07-29

Next message:
Subject: Re: estimate protein in Incl bod
From: Chun Luo chun {- at -} ACCELAGEN {- dot -} COM
Date: 2008-07-29

Subject: estimate protein in Incl bod
From: Meg mgbiotec {- at -} GMAIL {- dot -} COM
Date: 2008-07-29

We have our recombinant protein expressed in inclusion bodies we just wash
the IBs and perform solubilization and refolding. Our solubilisation protocol
mentions making up the volume to contain 7-8 mg / ml protein.

1. how can we determine the total protein content in the inclusion bodies
itself which are agregate mass . any good protocol if available.

2. we get our recombinant protein contaminated with host cell potein how to
get rid of the host cell proteins.

kindly suggest.

thanks and regards,

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: off-topic: protein crystallography in organic chemistry lab
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-07-29

Next message:
Subject: Re: estimate protein in Incl bod
From: Chun Luo chun {- at -} ACCELAGEN {- dot -} COM
Date: 2008-07-29