[ccp4bb] RES: [ccp4bb] Beamline Stability Issues

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Building NCS mate in Coot
From: Paul Emsley paul {- dot -} emsley {- at -} BIOCH {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2008-07-30

Next message:
Subject: Re: Preventing close contact between protein and ligand
From: Garib Murshudov garib {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2008-07-30

Subject: RES: Beamline Stability Issues
From: Lucas Sanfelici lsanfelici {- at -} LNLS {- dot -} BR
Date: 2008-07-30

Hi Michele!
-did you go in steps from 10 to 40 C? did you monitor drifts while doing
that? has the energy range, where you do not observe problems, changed?
In fact I was not planning to do that cause 40ºC
seems to be the mono’s basal temperature. In addition, I operated near
15ºC in the past and didn’t notice any appreciable change after that…
But I agree a more careful choice will be necessary in the near future!
-I am a bit worried about keeping the crystal at 40 C... did you speak
to the engineer who designed the mono about the optimal cooling
temperature?
Yes, but no kind of recommendation was informed in this way…

-which is actually your energy range now?
MX2 was projected to operate from 5 to 15 keV.
(http://rcsb-biosync-beta.rutgers.edu/lnls/W01B-MX2.html)
-do you have a compton scattering guard on the second crystal? We had
one, and it was surprising so see the marks that I had after a year of
being installed.
Ooh, unfortunately I don’t have one. I fear for
that.
-Check the water flow rate. Too high flow would cause turbulence, and a
too low, would not cool your crystal.
We are operating with about 0.8 liters/min. The
mono was projected to work with 1L/min.
do you have access to the machine data? electron beam position and so
on? this can help to find time correlation.
Yes I have. I’m starting to think what kind of
correlation would be elucidative…
Michele, thank you one more for your help. I’ll be pleased
to inform you about our progress.
Regards,
Lucas.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Building NCS mate in Coot
From: Paul Emsley paul {- dot -} emsley {- at -} BIOCH {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2008-07-30

Next message:
Subject: Re: Preventing close contact between protein and ligand
From: Garib Murshudov garib {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2008-07-30