[ccp4bb] protein or salt?

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: How to run SIGMAA twice in a row?
From: Huiying Li hli {- at -} CRYSTAL {- dot -} BIO {- dot -} UCI {- dot -} EDU
Date: 2007-04-18

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From: "Bussiere, Dirksen" dirksen {- dot -} bussiere {- at -} NOVARTIS {- dot -} COM
Date: 2007-04-18

Subject: protein or salt?
From: Noinaj noinaj {- at -} UKY {- dot -} EDU
Date: 2007-04-18

Hi,

i collected a diffraction pattern today of a protein crystal, hoping to answer the question, is it a protein or salt? The crystals appear as typical proteins crystals (from my experience), forming both plates and rods, easily crushed into millions of tiny crystals, and are bouyant. unfortunately, i collected a diffraction pattern that is a bit confusing. you can see the image at the following URL:

http://www.noinaj.com/temp/screen1.jpg

while i don't see very low resolutions spots, i do see spots lower than 4 ang; it seems nothing is available for lower resolution than that. does this indicate salt (despite properties screening), which I thought would only show high resolution spots, and only a few, not as many as observed in the image.

Essentially, could anyone offer any further analysis of the diffraction pattern and help me decide whether this is a protein or salt? Thanks in advance for all feedback!!!

Cheers,
Nick

________________________________________

Nicholas Noinaj
University of Kentucky School of Medicine
Department of Molecular and Cellular Biochemistry
Biomedical Biological Sciences Research Building, Rm 236
741 S. Limestone
Lexington, Ky 40536
Lab: 859-323-8183
Home: 859-228-0978
noinaj@uky.edu
www.noinaj.com

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: How to run SIGMAA twice in a row?
From: Huiying Li hli {- at -} CRYSTAL {- dot -} BIO {- dot -} UCI {- dot -} EDU
Date: 2007-04-18