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[ccp4bb] chromophore in GFP
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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
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From: "William G {- dot -} Scott" wgscott {- at -} CHEMISTRY {- dot -} UCSC {- dot -} EDU
Date: 2008-08-01


Subject: chromophore in GFP
From: sheng CUI sheng {- at -} LMB {- dot -} UNI-MUENCHEN {- dot -} DE
Date: 2008-08-01

Hi everybody,



we have solved the X-ray structure of a GFP (green fluorescent protein)
containing complex. During refinement of the structure we now encountered
the problem of connecting the GFP chromophore covalently to the polypeptide
chain. We introduced the chromophore to our model as a separate ligand with
separate toplogy. So far we could not find a way to integrate it in the
primary sequence.



Any idea how this problem can be solved? Which programs might be useful?



Thank you very much in advance.



Best,



Sheng & Axel





*************************************************************************



Dr. Sheng CUI ´Þʤ



Department of Chemistry and Biochemistry
Gene Center
University of Munich
Feodor-Lynen-Str. 25
81377 Munich, Germany



Tel: +49 89 2180-76984
Fax: +49 89 2180-76999
e-mail: sheng@lmb.uni-muenchen.de

Web:
http://www.lmb.uni-muenchen.de/hopfner/welcome.html



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