Re: [ccp4bb] chromophore in GFP

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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From: James Stroud jstroud {- at -} MBI {- dot -} UCLA {- dot -} EDU
Date: 2008-08-02

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From: Sun Tang suntang2222 {- at -} YAHOO {- dot -} COM
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Subject: Re: chromophore in GFP
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-08-02

Defining specific restraints on bonds connecting the chromophore with the
rest of the protein worked fine for us in Refmac when we solved a turboGFP
structure a couple of years ago. There wasn¡¯t anything special that had to
be done ¨C all strictly by the book¡

Artem

_____

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of sheng
CUI
Sent: Friday, August 01, 2008 7:32 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] chromophore in GFP

Hi everybody,

we have solved the X-ray structure of a GFP (green fluorescent protein)
containing complex. During refinement of the structure we now encountered
the problem of connecting the GFP chromophore covalently to the polypeptide
chain. We introduced the chromophore to our model as a separate ligand with
separate toplogy. So far we could not find a way to integrate it in the
primary sequence.

Any idea how this problem can be solved? Which programs might be useful?

Thank you very much in advance.

Best,

Sheng & Axel

*************************************************************************

Dr. Sheng CUI ´ÞÊ¤

Department of Chemistry and Biochemistry
Gene Center
University of Munich
Feodor-Lynen-Str. 25
81377 Munich, Germany

Tel: +49 89 2180-76984
Fax: +49 89 2180-76999
e-mail: sheng@lmb.uni-muenchen.de

Web:
http://www.lmb.uni-muenchen.de/hopfner/welcome.html

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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Pymol movie ration along any axis
From: James Stroud jstroud {- at -} MBI {- dot -} UCLA {- dot -} EDU
Date: 2008-08-02

Next message:
Subject: Re: question about getting rid of model bias in refinement
From: Sun Tang suntang2222 {- at -} YAHOO {- dot -} COM
Date: 2008-08-02