[ccp4bb] protein crystal decay problem

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: R-axis HTC set of images .. got some
From: Andrew Leslie andrew {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2008-08-04

Next message:
Subject: Re: protein crystal decay problem
From: junfeng liu jliu {- at -} NIMR {- dot -} MRC {- dot -} AC {- dot -} UK
Date: 2008-08-04

Subject: protein crystal decay problem
From: lei feng spartanfenglei {- at -} HOTMAIL {- dot -} COM
Date: 2008-08-04

hello everyone, I am seeking help on a crystal decay problem

I got crystals of a protein , but it decayed very fast in syncrotron beam. From the initial index, it is P222 space group, I need about 180 images, its initial diffraction went to about 3 A, and after 70-80 images, it droped to about 6. I already used low exposure time and it is in cryo , what I can do ? any suggestion is appreciated.

BTW. the condition of crystallization is 0.1M NaAC, pH 5.5, 1 M LiCl, 15% PEG 6000,

I used 5 seconds / image , which can go to 3 A, if I use 2 seconds, the initial resolution is only 3.5 A ( beam energy 12kev)

Thanks
_________________________________________________________________
Got Game? Win Prizes in the Windows Live Hotmail Mobile Summer Games Trivia Contest
http://www.gowindowslive.com/summergames?ocid=TXT_TAGHM

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: R-axis HTC set of images .. got some
From: Andrew Leslie andrew {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2008-08-04

Next message:
Subject: Re: protein crystal decay problem
From: junfeng liu jliu {- at -} NIMR {- dot -} MRC {- dot -} AC {- dot -} UK
Date: 2008-08-04