Re: [ccp4bb] protein crystal decay problem

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: protein crystal decay problem
From: lei feng spartanfenglei {- at -} HOTMAIL {- dot -} COM
Date: 2008-08-04

Next message:
Subject: Re: protein crystal decay problem
From: Juergen Bosch jbosch {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2008-08-04

Subject: Re: protein crystal decay problem
From: junfeng liu jliu {- at -} NIMR {- dot -} MRC {- dot -} AC {- dot -} UK
Date: 2008-08-04

How about 90?
If it is ok , maybe you need more than 3 or more xtals to collect the
whole dataset and merge them together.
Otherwise your xtals should be the anisotropic .You'd better to collect
images in the better region or screen more xtals to get better ones.
Good luck!
leo
lei feng wrote:
> hello everyone, I am seeking help on a crystal decay problem
>
> I got crystals of a protein , but it decayed very fast in syncrotron
> beam. From the initial index, it is P222 space group, I need about 180
> images, its initial diffraction went to about 3 A, and after 70-80
> images, it droped to about 6. I already used low exposure time and it
> is in cryo , what I can do ? any suggestion is appreciated.
>
> BTW. the condition of crystallization is 0.1M NaAC, pH 5.5, 1 M
> LiCl, 15% PEG 6000,
>
> I used 5 seconds / image , which can go to 3 A, if I use 2 seconds,
> the initial resolution is only 3.5 A ( beam energy 12kev)
>
> Thanks
>
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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: protein crystal decay problem
From: lei feng spartanfenglei {- at -} HOTMAIL {- dot -} COM
Date: 2008-08-04

Next message:
Subject: Re: protein crystal decay problem
From: Juergen Bosch jbosch {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2008-08-04