Re: [ccp4bb] protein or salt?

Protein crystallography Programs for crystallography X-ray detectors
Basic tutorials:
Protein Peptide

CCP4bb navigation

CCP4bb <-- 2007 <-- April 2007 <-- 18 April 2007

Previous message:
Subject: Re: protein or salt?
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2007-04-18

Next message:
Subject: Protein expression in Minimal media (M9)
From: mshah {- at -} HWI {- dot -} BUFFALO {- dot -} EDU mshah {- at -} HWI {- dot -} BUFFALO {- dot -} EDU
Date: 2007-04-18

Subject: Re: protein or salt?
From: Frank von Delft frank {- dot -} vondelft {- at -} SGC {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2007-04-18

Hi Nick, it's not obvious to me this is a single lattice, hence the "many"
spots. But it's not protein, don't worry about that.

phx.

_____

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Noinaj
Sent: 19 April 2007 00:19
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] protein or salt?

Hi,

i collected a diffraction pattern today of a protein crystal, hoping to
answer the question, is it a protein or salt? The crystals appear as
typical proteins crystals (from my experience), forming both plates and
rods, easily crushed into millions of tiny crystals, and are bouyant.
unfortunately, i collected a diffraction pattern that is a bit confusing.
you can see the image at the following URL:

http://www.noinaj.com/temp/screen1.jpg

while i don't see very low resolutions spots, i do see spots lower than 4
ang; it seems nothing is available for lower resolution than that. does
this indicate salt (despite properties screening), which I thought would
only show high resolution spots, and only a few, not as many as observed in
the image.

Essentially, could anyone offer any further analysis of the diffraction
pattern and help me decide whether this is a protein or salt? Thanks in
advance for all feedback!!!

Cheers,
Nick

________________________________________

Nicholas Noinaj
University of Kentucky School of Medicine
Department of Molecular and Cellular Biochemistry
Biomedical Biological Sciences Research Building, Rm 236
741 S. Limestone
Lexington, Ky 40536
Lab: 859-323-8183
Home: 859-228-0978
noinaj@uky.edu
www.noinaj.com

CCP4bb navigation

CCP4bb <-- 2007 <-- April 2007 <-- 18 April 2007

Previous message:
Subject: Re: protein or salt?
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2007-04-18

Next message:
Subject: Protein expression in Minimal media (M9)
From: mshah {- at -} HWI {- dot -} BUFFALO {- dot -} EDU mshah {- at -} HWI {- dot -} BUFFALO {- dot -} EDU
Date: 2007-04-18