- Protein crystallography
Main steps: - Protein purification - Crystallisation
Special: - Programs for crystallography - X-ray detectors
Basic tutorials: - Chemistry - Protein - Peptide - Amino Acids
Xtal community: - CCP4BB
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Subject: Re: Protein expression in Minimal media (M9) From: "Richard P {- dot -} Grant" rpg {- at -} USYD {- dot -} EDU {- dot -} AU Date: 2007-04-18
Hi,
from my experience with minimal media expression you're probably better off starting with a good, thick rich media overnight growth, and then diluting that out 1:20 – 1:50 into the M9.
Richard
-- Richard P. Grant School of Molecular & Microbial Biosciences University of Sydney x14025/17817 PGP:0x9F5687DB
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