Re: [ccp4bb] Protein expression in Minimal media (M9)

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

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   - Protein
   - Peptide
   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Protein expression in Minimal media (M9)
From: mshah {- at -} HWI {- dot -} BUFFALO {- dot -} EDU mshah {- at -} HWI {- dot -} BUFFALO {- dot -} EDU
Date: 2007-04-18

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Subject: Summary - Stop Refmac from refining B factors?
From: Eva Kirchner eva {- dot -} kirchner {- at -} UNI-TUEBINGEN {- dot -} DE
Date: 2007-04-19

Subject: Re: Protein expression in Minimal media (M9)
From: "Richard P {- dot -} Grant" rpg {- at -} USYD {- dot -} EDU {- dot -} AU
Date: 2007-04-18

Hi,

from my experience with minimal media expression you're probably
better off starting with a good, thick rich media overnight growth,
and then diluting that out 1:20 – 1:50 into the M9.

Richard

--
Richard P. Grant
School of Molecular & Microbial Biosciences University of Sydney
x14025/17817 PGP:0x9F5687DB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Protein expression in Minimal media (M9)
From: mshah {- at -} HWI {- dot -} BUFFALO {- dot -} EDU mshah {- at -} HWI {- dot -} BUFFALO {- dot -} EDU
Date: 2007-04-18

Next message:
Subject: Summary - Stop Refmac from refining B factors?
From: Eva Kirchner eva {- dot -} kirchner {- at -} UNI-TUEBINGEN {- dot -} DE
Date: 2007-04-19