Quick navigation: Home   |    Site Map   ||    References   |    Biography   ||    Copyright   |    Other copyright   |    Contact us   |    Advert   |   
 

[ccp4bb] I/sigma in XDS output

- Protein crystallography

Main steps:

   - Protein purification
   - Crystallisation

Special:

   - Programs for crystallography
   - X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

   - CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: Protein expression in Minimal media (M9)
From: Cynthia Kinsland clk10 {- at -} CORNELL {- dot -} EDU
Date: 2007-04-19
Next message:
Subject: MSc in Structural Biology and Biophysics at Manchester, UK
From: Martyn Winn m {- dot -} d {- dot -} winn {- at -} DL {- dot -} AC {- dot -} UK
Date: 2007-04-19


Subject: I/sigma in XDS output
From: Michele Lunelli efunit {- at -} YAHOO {- dot -} IT
Date: 2007-04-19

Dear all,
I'm a bit confused from the output of the CORRECT step in XDS. In one of the
first tables I can read the mean I/sigma for each resolution shell, but these
values are much different from the I/sigma reported in the table at the end of
the output files, titled "completeness and quality of data set" for the full
data range with signal/noise > -3.0. For example, from the first table I have
I/sigma = 2 at 3.6 A, while from the second table I have I/sigma = 2 at 2.8 A!
What is exactly the difference between the two values? And which one is reliable
to decide the resolution cutoff?


Thank you in advance,

Michele Lunelli
MPI for Infection Biology
Berlin - Germany








----

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: Protein expression in Minimal media (M9)
From: Cynthia Kinsland clk10 {- at -} CORNELL {- dot -} EDU
Date: 2007-04-19
Next message:
Subject: MSc in Structural Biology and Biophysics at Manchester, UK
From: Martyn Winn m {- dot -} d {- dot -} winn {- at -} DL {- dot -} AC {- dot -} UK
Date: 2007-04-19



ProteinCrystallography.org: Copyright 2006-2010 by Quid United Ltd