[ccp4bb] Lower completeness, decent R factors, but low B factor...

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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From: Sangeetha Vedula sangeetha {- dot -} bb {- at -} GMAIL {- dot -} COM
Date: 2008-08-20

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Date: 2008-08-20

Subject: Lower completeness, decent R factors, but low B factor...
From: James Pauff pauffjm {- at -} YAHOO {- dot -} COM
Date: 2008-08-20

Hello all,

I have a refined structure at 2.6 angstroms that at about 73% completeness at this resolution. The I/sigma is about 2.0 at 2.6 angstroms, and the omit density for my ligands is great contoured at 3.0sigma. My Rcryst is 19 or so and the Rfree is 24.5 or so.

HOWEVER, my mean B value is 13.9, whereas my other 2 structures (at 2.2 and 2.3 angstroms, same protein, >95% completeness) have mean B values of 22+. Any suggestions as to what is going on here? I'm having trouble explaining this.

Thank you,
Jim

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: VOIDOO average volume report
From: Sangeetha Vedula sangeetha {- dot -} bb {- at -} GMAIL {- dot -} COM
Date: 2008-08-20