Re: [ccp4bb] Lower completeness, decent R factors, but low B factor...

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: libcheck error
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2008-08-20

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Subject: Re: Lower completeness, decent R factors, but low B factor...
From: Anastassis Perrakis a {- dot -} perrakis {- at -} NKI {- dot -} NL
Date: 2008-08-20

Subject: Re: Lower completeness, decent R factors, but low B factor...
From: Wim Burmeister wpb {- at -} EMBL {- dot -} FR
Date: 2008-08-20

James Pauff a écrit :
> Hello all,
>
> I have a refined structure at 2.6 angstroms that at about 73% completeness at this resolution. The I/sigma is about 2.0 at 2.6 angstroms, and the omit density for my ligands is great contoured at 3.0sigma. My Rcryst is 19 or so and the Rfree is 24.5 or so.
>
> HOWEVER, my mean B value is 13.9, whereas my other 2 structures (at 2.2 and 2.3 angstroms, same protein, >95% completeness) have mean B values of 22+. Any suggestions as to what is going on here? I'm having trouble explaining this.
>
> Thank you,
> Jim
>
>
>
>
>
>
Dear Jim,

probably you did not collect your data to the highest possible
resolution. Did you use an inhouse source? You would expect that a
crystal with an average temperature factor of 14 A2 would diffract to
1.6 A on a synchrotron source.

Regards

Wim

--
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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: libcheck error
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2008-08-20

Next message:
Subject: Re: Lower completeness, decent R factors, but low B factor...
From: Anastassis Perrakis a {- dot -} perrakis {- at -} NKI {- dot -} NL
Date: 2008-08-20