Re: [ccp4bb] Running Resolve after Sharp?

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Crystallization position at Abbott Laboratories - still open
From: Russell A Judge russell {- dot -} judge {- at -} ABBOTT {- dot -} COM
Date: 2008-08-22

Next message:
Subject: Re: How best to scale together absolute and Fo-Fc density maps?
From: Ian Tickle I {- dot -} Tickle {- at -} ASTEX-THERAPEUTICS {- dot -} COM
Date: 2008-08-22

Subject: Re: Running Resolve after Sharp?
From: artem {- at -} XTALS {- dot -} ORG artem {- at -} XTALS {- dot -} ORG
Date: 2008-08-22

Yes, I did that recently and it worked although I found SOLOMON maps to be
(subjectively) better. You should probably use the 'centroid' phases from
the eden.mtz (SHARP gurus might want to correct me :))
Artem

> Hi
>
> Sorry for a mixed up question about two great software.
>
> Has anybody tried to use Resolve after sharp / autosharp? i.e.,
> escaping the Solomon step other than handedness determination and
> doing the density modification (& maybe NCS / building) directly with
> the sharp output? If so, I would appreciate any input.. I am confused
> with the eden file..
>
> Cheers, Partha
>
>
>
>
>
> --
> MRC National Institute for Medical Research
> Division of Molecular Structure
> The Ridgeway, NW7 1AA, UK
> Email: pchakra@nimr.mrc.ac.uk
> Phone: + 44 208 816 2515
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Crystallization position at Abbott Laboratories - still open
From: Russell A Judge russell {- dot -} judge {- at -} ABBOTT {- dot -} COM
Date: 2008-08-22

Next message:
Subject: Re: How best to scale together absolute and Fo-Fc density maps?
From: Ian Tickle I {- dot -} Tickle {- at -} ASTEX-THERAPEUTICS {- dot -} COM
Date: 2008-08-22