[ccp4bb] exposing your Tev site

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

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   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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From: o {- dot -} h {- dot -} weiergraeber {- at -} FZ-JUELICH {- dot -} DE o {- dot -} h {- dot -} weiergraeber {- at -} FZ-JUELICH {- dot -} DE
Date: 2007-04-20

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Subject: exposing your Tev site
From: "Lukacs, Christine" christine {- dot -} lukacs {- at -} ROCHE {- dot -} COM
Date: 2007-04-20

Quick question about Tev sites -
We have a situation where our N-terminal TEV site is inaccessible to the
protease. Looking at a close homolog, we realized that our first
protein residue is already involved in a beta sheet. From peoples'
experience - how FEW residues can we add between the cleavage site and
the first protein residue in order to make the cleavage site accessible
to the protease for efficient cleavage of the tag? (Tagged protein
behaves well but crystallizes poorly, we are hoping that cleavage of the
tag will improve things)

Thanks for any anecdotes-

Christine Lukacs
Roche

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