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[ccp4bb] exposing your Tev site

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: further inconsistencies (was: tcltkblt and python distributions)
From: o {- dot -} h {- dot -} weiergraeber {- at -} FZ-JUELICH {- dot -} DE o {- dot -} h {- dot -} weiergraeber {- at -} FZ-JUELICH {- dot -} DE
Date: 2007-04-20
Next message:
Subject: Re: recommendation for heavy atoms used in lithium sulfate and ammonium sulfate
From: Jeffrey {- dot -} Kieft {- at -} UCHSC {- dot -} EDU Jeffrey {- dot -} Kieft {- at -} UCHSC {- dot -} EDU
Date: 2007-04-20


Subject: exposing your Tev site
From: "Lukacs, Christine" christine {- dot -} lukacs {- at -} ROCHE {- dot -} COM
Date: 2007-04-20

Quick question about Tev sites -
We have a situation where our N-terminal TEV site is inaccessible to the
protease. Looking at a close homolog, we realized that our first
protein residue is already involved in a beta sheet. From peoples'
experience - how FEW residues can we add between the cleavage site and
the first protein residue in order to make the cleavage site accessible
to the protease for efficient cleavage of the tag? (Tagged protein
behaves well but crystallizes poorly, we are hoping that cleavage of the
tag will improve things)

Thanks for any anecdotes-

Christine Lukacs
Roche


CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: further inconsistencies (was: tcltkblt and python distributions)
From: o {- dot -} h {- dot -} weiergraeber {- at -} FZ-JUELICH {- dot -} DE o {- dot -} h {- dot -} weiergraeber {- at -} FZ-JUELICH {- dot -} DE
Date: 2007-04-20
Next message:
Subject: Re: recommendation for heavy atoms used in lithium sulfate and ammonium sulfate
From: Jeffrey {- dot -} Kieft {- at -} UCHSC {- dot -} EDU Jeffrey {- dot -} Kieft {- at -} UCHSC {- dot -} EDU
Date: 2007-04-20



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