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Re: [ccp4bb] recommendation for heavy atoms used in lithium sulfate and ammonium sulfate
 

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CCP4bb <-- 2007 <-- April 2007 <-- 20 April 2007
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Subject: Re: exposing your Tev site
From: Sebastien Violot svicri {- at -} IBMB {- dot -} CSIC {- dot -} ES
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Date: 2007-04-20


Subject: Re: recommendation for heavy atoms used in lithium sulfate and ammonium sulfate
From: artem {- at -} XTALS {- dot -} ORG artem {- at -} XTALS {- dot -} ORG
Date: 2007-04-20
Tiancen

If you have enough crystals to experiment with, just do a normal series of
heavy atom derivatives, starting with good old favorites such as mercury
and platinum. As an added touch of class you can try tungstate - it's like
sulphate, but has fat W so it can be used probably just like a halide
soak...

Let me know if you need more detailed help :)

Artem

> Dear all,
>
> Could any one recommend some heavy atoms used for crystals grown in 0.1M
> tri-sodium citrate pH 5.6, 1M Lithium sulfate and 0.5M ammonium sulfate? I
> read from Hampton user guide of heavy atom kit that "high salt
> concentrations are not the ideal medium for heavy atom reactions with
> macromolecules", so is there any type of heavy atom we should avoid using?
> We do not have any experience in preparing heavy atom derivative, so any
> suggestions, experience or references will be greatly appreciated.
>
> Thanks in advance!
>
> Tiancen Hu
> Shanghai Institute of Materia Medica
>

CCP4bb navigation

CCP4bb <-- 2007 <-- April 2007 <-- 20 April 2007
Previous message:
Subject: Re: exposing your Tev site
From: Sebastien Violot svicri {- at -} IBMB {- dot -} CSIC {- dot -} ES
Date: 2007-04-20 		Next message:
Subject: Re: How to run SIGMAA twice in a row? (fwd)
From: Peter Adrian Meyer pam52 {- at -} CORNELL {- dot -} EDU
Date: 2007-04-20


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