Re: [ccp4bb] regarding cloning

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Como Crystallography School registration deadline extended for the last time!
From: Derek Logan Derek {- dot -} Logan {- at -} MBFYS {- dot -} LU {- dot -} SE
Date: 2008-09-01

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Subject: Re: regarding cloning
From: Roger Rowlett rrowlett {- at -} MAIL {- dot -} COLGATE {- dot -} EDU
Date: 2008-09-01

Subject: Re: regarding cloning
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-09-01

T/A cloning utilizes the overhangs left by certain polymerases as cloning
handles. To lower background, the vectors for TA cloning are often designed
to contain a rare cutter which is used during ligation to constantly re-cut
the self-ligated vector, so this way only the insert ligation product is
supposed to survive.

A.

-----Original Message-----
I have heard of TOPO-TA cloning. Not sure what T/A cloning is.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Next message:
Subject: Re: regarding cloning
From: Roger Rowlett rrowlett {- at -} MAIL {- dot -} COLGATE {- dot -} EDU
Date: 2008-09-01