Re: [ccp4bb] Protein Color

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

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   - Peptide
   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Protein Color
From: Roger Rowlett rrowlett {- at -} MAIL {- dot -} COLGATE {- dot -} EDU
Date: 2008-09-05

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Subject: pKa for protein C-terminus?
From: Patrick Loll pat {- dot -} loll {- at -} DREXEL {- dot -} EDU
Date: 2008-09-05

Subject: Re: Protein Color
From: Kornelius Zeth kornelius {- dot -} zeth {- at -} TUEBINGEN {- dot -} MPG {- dot -} DE
Date: 2008-09-05

you can do a simple wavelength scan at the synchrotron of the protein solution frozen in a loop.

Best wishes

Kornelius

On Fri, 5 Sep 2008 12:21:29 -0400
Matthew Alan Bratkowski wrote:
> Hello.
>
> I am working with a protein that turns a yellowish-brown color when it is
> concentrated to around 2 mg/ml or higher in a small volume (a few hundred
> uL). I was wondering if the protein bound a metal or other prosthetic
> group that would give it this color? The protein's color somewhat
> resembles iron binding proteins, but there is no peak in the 400 nm range
> that would suggest heme, and an iron sulfur cluster is not that likely
> since there are only five cysteines in the protein. Proteins with
> structures homologous to the one I am studying bind magnesium, but are not
> know to bind other metals. Any information about what this color might
> suggest about the protein or how I could analyze possible bound metals or
> prosthetic groups using only a small amount of protein would be helpful.
>
> Matt

----------------------------------------------
Kornelius Zeth
Max Planck Institute for Developmental Biology
Dept. Protein Evolution
Spemannstr. 35
72076 Tuebingen, Germany
kornelius.zeth@tuebingen.mpg.de
Tel -49 7071 601 323
Fax -49 7071 601 349

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Protein Color
From: Roger Rowlett rrowlett {- at -} MAIL {- dot -} COLGATE {- dot -} EDU
Date: 2008-09-05

Next message:
Subject: pKa for protein C-terminus?
From: Patrick Loll pat {- dot -} loll {- at -} DREXEL {- dot -} EDU
Date: 2008-09-05