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Re: [ccp4bb] Ecoli protein staining with AntiHis? |
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CCP4bb navigationCCP4bb <-- 2007 <-- April 2007 <-- 20 April 2007Subject: Re: Ecoli protein staining with AntiHis? From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG Date: 2007-04-20 light up different stuff. We very rarely see just one band - typically it's a bunch of weak bands as well as smears. How strong is the band? Can you cut it out of the gel and do a tryptic digest and MS identification? If the band is strong, consider the possibility that you've somehow contaminated your strain with some other expressor... Artem -----Original Message----- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Raji Edayathumangalam Sent: Friday, April 20, 2007 4:36 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Ecoli protein staining with AntiHis? Hi Everyone, I see a band that lights up in my anti-His Western blots. While I investigate what else the band might be (truncated protein etc.), does anyone know whether there is an E. coli protein that migrates ~ 30-40kDa (SDS-PAGE), which binds to anti-His antibodies? Thanks. Raji CCP4bb navigationCCP4bb <-- 2007 <-- April 2007 <-- 20 April 2007 |
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