Re: [ccp4bb] crystal size

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: xds and Saturn troubles
From: Jan Abendroth jan {- dot -} abendroth {- at -} GMAIL {- dot -} COM
Date: 2008-09-10

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Subject: Re: crystal size
From: "William G {- dot -} Scott" wgscott {- at -} CHEMISTRY {- dot -} UCSC {- dot -} EDU
Date: 2008-09-10

Subject: Re: crystal size
From: Nurit Mirkin nuritmirkin {- at -} HOTMAIL {- dot -} COM
Date: 2008-09-10

Hi!
I would suggest to try another temperature or seeding. It worked for me before.
Good luck!
Nurit.

"Anyone who has never made a mistake has never tried anything new. Albert Einstein"
Nurit Mirkin Bril> Date: Wed, 10 Sep 2008 08:02:53 -0400> From: rrowlett@MAIL.COLGATE.EDU> Subject: Re: [ccp4bb] crystal size> To: CCP4BB@JISCMAIL.AC.UK> > My usual first approach to increasing crystal size is to decrease > precipitant and increase protein concentration, keeping the mixture in > the proper nucleation zone, but providing more protein for crystal > growth. Minimizing dust or particulates by filtration and centrifugation > of reagents and protein may help, by reducing nucleation sites in the > solution. Crystallization at a lower temperature may also result in > fewer, but larger crystals. Or you could try seeding.> > Cheers,> > -- > ------------------------------------------------------------------------> Roger S. Rowlett> Professor> Colgate University Presidential Scholar> Department of Chemistry> Colgate University> 13 Oak Drive> Hamilton, NY 13346> > tel: (315)-228-7245> ofc: (315)-228-7395> fax: (315)-228-7935> email: rrowlett@mail.colgate.edu> > > Walter Novak wrote:> > Hi Vince,> >> > There is a very nice paper by Monika Budayova-Spano in Acta Cryst 63D > > 2007 339-347. "A methodology and an instrument for the > > temperature-controlled optimization of crystal growth." > >> > Best,> > Wally> >> > On Sep 10, 2008, at 2:23 AM, Vincenzo Carbone wrote:> >> >> Dear all,> >>> >> > >>> >> I was wondering if anyone had some practical advice in regards to > >> increasing the size of a crystal. Currently my enzyme forms these > >> rather nice cubic and very sharp <0.1mm (in 25% peg 5000, 0.1M > >> ammonium sulphate, 0.1M Tris ph 7.0) crystals following optimisation > >> of the pH and precipitant concentrations of the initial screening > >> condition and by playing with various increasing levels of protein > >> concentration 20-60mg/ml.> >>> >> > >>> >> Has anyone had success with additive screens in increasing crystal > >> size and what were they?> >>> >> > >>> >> Any advice would be useful thanks> >>> >> > >>> >> Vince> >> >> > Walter R.P. Novak, Ph.D.> > Postdoctoral Fellow> > Rosenstiel Basic Medical Research Center> > Brandeis University> > 415 South St. MS 029> > Waltham, MA 02454-9110> > Phone: (781) 736-4944> > Fax: (781) 736-2405> >> >> >> >> > -
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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: xds and Saturn troubles
From: Jan Abendroth jan {- dot -} abendroth {- at -} GMAIL {- dot -} COM
Date: 2008-09-10

Next message:
Subject: Re: crystal size
From: "William G {- dot -} Scott" wgscott {- at -} CHEMISTRY {- dot -} UCSC {- dot -} EDU
Date: 2008-09-10