Re: [ccp4bb] process SeMet labelled data

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Cannot running NTA to purify the protein having His-tag?
From: artem {- at -} XTALS {- dot -} ORG
Date: 2007-03-01

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From: timmins {- at -} ILL {- dot -} FR
Date: 2007-03-01

Subject: Re: process SeMet labelled data
From: ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-03-01

shivesh kumar wrote:
> Dear all,
> I have a data set at 2.2A, of the selenomethionene labelled
> protein.How should I process the data.Thanx for the help.
> Shivesh

I think you should ask your local crystallographer for help. There are
several programs ( MOSFLM in CCP4, DENZO, XDS, D*TREK ) but you will
need some guideance to find them and get started.
Eleanor

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Cannot running NTA to purify the protein having His-tag?
From: artem {- at -} XTALS {- dot -} ORG
Date: 2007-03-01

Next message:
Subject: FW: Vacancy in Small Angle Neutron Scattering at ILL, Grenoble.
From: timmins {- at -} ILL {- dot -} FR
Date: 2007-03-01