[ccp4bb] protein complex crystallisation

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Postdoctoral position in Michael Rossmann's laboratory
From: Petr Leiman leiman {- at -} PURDUE {- dot -} EDU
Date: 2008-09-20

Next message:
Subject: Re: protein complex crystallisation
From: "Radisky, Evette S {- dot -} , Ph {- dot -} D {- dot -} " Radisky {- dot -} Evette {- at -} MAYO {- dot -} EDU
Date: 2008-09-20

Subject: protein complex crystallisation
From: Ron hudson hudson {- dot -} genome {- at -} GMAIL {- dot -} COM
Date: 2008-09-20

Dear CCP4 Community,

My apologies for the off topic question to the bb.

I am trying to crystallise a small protein-protein complex. I purify as a
complex protein after expression in BL21DE3 cells through NI-NTA affinity
chromatography. pI of one of the protein is around 10.6 where as another
component have 4.0. I am in the screening stage of the crystallisation.
During crystallistion process it precipitate very quickly as i add the
protein into the crytallisation solution drop. This happens in almost all
the condition of the hampton INDEX and crystal screens. I purify this
complex in Tris-Cl buffer at pH=8.0. it is happily soluble and donot
prcipitate at this pH in the same buffer. I can concetrate this protein upto
~10-15mg/ml. could any one suggest the solution, that will be most
appreciatable.

Thanks in advance

Ron

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Postdoctoral position in Michael Rossmann's laboratory
From: Petr Leiman leiman {- at -} PURDUE {- dot -} EDU
Date: 2008-09-20

Next message:
Subject: Re: protein complex crystallisation
From: "Radisky, Evette S {- dot -} , Ph {- dot -} D {- dot -} " Radisky {- dot -} Evette {- at -} MAYO {- dot -} EDU
Date: 2008-09-20