Re: [ccp4bb] Quick-soak

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Off-topic: coomassie linearity?
From: Juergen Bosch jbosch {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2008-09-26

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Subject: Re: Quick-soak
From: David Briggs drdavidcbriggs {- at -} GMAIL {- dot -} COM
Date: 2008-09-26

Hi Amit,

http://www.doe-mbi.ucla.edu/~sawaya/tutorials/Phasing/references.html

And the (IMHO) seminal heavy atom derivative reference:

Petsko, G.A., "Perparation of Isomorphous Heavy-Atom Derivatives"
Methods in Enzymology, Volume 114, , pages 147-157.

should give you all the info you need.

The 0.15M KBr might give you a usable anomalous signal, but the only
way you'll be able to determine that is to go ahead and do it!
the f" of Br @ CuKa is ~1.2e-, with careful data collection, you might
get lucky.

Cheers,

David

2008/9/25 amit sharma <3112amit@gmail.com>:
>
> Dear CCP4bbers,
> I have a protein molecule(~9.0 kDa) that crystallized in the presence of
> 0.15M KBr and HEPES pH 7.0. Since there is no homologuous structure present,
> I intend to perform heavy metal derivatization. I read some literature which
> suggested that I could carry out quick soak with 0.5M Sodium iodide. I was
> wondering if I could soak my crystals with a similar concentration of NaBr
> and collect some low resolution data at the in-house source? In addition
> should I try to also introduce other heavy metals? it might be worth
> mentioning that my protein carries no methionine/cys residues.
> I have no prior experience in doing this. So, it would be of great help if
> I could be directed towards literature/protocols pertaining to this. Any
> advice/suggestions would be greatly appreciated.
>
> Thanks in advance
> --
> Amit Sharma

--
============================
David C. Briggs PhD
Father & Crystallographer
http://drdavidcbriggs.googlepages.com/home
AIM ID: dbassophile
============================

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Off-topic: coomassie linearity?
From: Juergen Bosch jbosch {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2008-09-26

Next message:
Subject: Post Doctoral position available in protein engineering at Lonza-APT
From: Lang Dietmar - Slough dietmar {- dot -} lang {- at -} LONZA {- dot -} COM
Date: 2008-09-26