Re: [ccp4bb] Off-topic: coomassie linearity?

- Protein crystallography

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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From: "George M {- dot -} Sheldrick" gsheldr {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2008-09-26

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Subject: Re: Off-topic: coomassie linearity?
From: Gregory Alushin galushin {- at -} BERKELEY {- dot -} EDU
Date: 2008-09-26

The Bio-Rad Flamingo Fluorescent Gel Stain has a very linear profile
with protein concentration (if you believe the standard curves in the
manual). In my hands it gives nice results for binding assays.

Cheers,
-Greg Alushin

On Sep 25, 2008, at 2:35 PM, Jacob Keller wrote:

> Dear Crystallographers,
>
> I remember having seen on this listserve that coomassie stain is
> horribly non-linear in intensity per protein concentration, which
> leads me to two questions:
>
> 1. Does anybody have a reference for quantitation of coomassie's
> linearity (and possibly other stains), i.e. where and how extensive
> the linear range is, and
> 2. Can anybody suggest a more linear protein gel stain?
>
> Thanks,
>
> Jacob Keller
>
> *******************************************
> Jacob Pearson Keller
> Northwestern University
> Medical Scientist Training Program
> Dallos Laboratory
> F. Searle 1-240
> 2240 Campus Drive
> Evanston IL 60208
> lab: 847.491.2438
> cel: 773.608.9185
> email: j-keller2@northwestern.edu
> *******************************************

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Next message:
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Date: 2008-09-26