Re: [ccp4bb] precipitates

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: mtz library in windows
From: Alex XIE itn2000uk {- at -} GMAIL {- dot -} COM
Date: 2008-09-30

Next message:
Subject: Re: precipitates
From: amit sharma 3112amit {- at -} GMAIL {- dot -} COM
Date: 2008-10-01

Subject: Re: precipitates
From: Chavas Leo ccp4hnaalas {- at -} GMAIL {- dot -} COM
Date: 2008-10-01

Dear Amit --

On 30 Sep 2008, at 14:16, amit sharma wrote:
> I am trying to crystallize a protein with a peptide attached to it
> via a 9aa linker. I am mostly getting precipitates in conditions
> carrying salts. I have tried optimization screens by varying the pH
> and salt concentrations, but in vain. Can anyone please suggest
> ways by which I could optimize the conditions further?

Among all the possibilities, you can:
- try to crystallize without salt ;
- change the temperature ;
- dilute your protein ;
- add some isopropanol (or other) to make the solution "smoother" ;
- change your linker size ;
- ...

HTH
Kind regards.

-- Leo --
------------------------------------------------------------
Chavas Leonard, Ph.D. @ home
Research Associate
Marie Curie Actions Fellow
------------------------------------------------------------
Faculty of Life Sciences
The University of Manchester
The Michael Smith Building
Oxford Road
Manchester Lancashire
M13 9PT
------------------------------------------------------------
Tel: +44(0)161-275-1586
e-mail: Leonard.Chavas@manchester.ac.uk
http://personalpages.manchester.ac.uk/staff/leonard.chavas/

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: mtz library in windows
From: Alex XIE itn2000uk {- at -} GMAIL {- dot -} COM
Date: 2008-09-30

Next message:
Subject: Re: precipitates
From: amit sharma 3112amit {- at -} GMAIL {- dot -} COM
Date: 2008-10-01