Re: [ccp4bb] precipitates

- Protein crystallography

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: precipitates
From: Chavas Leo ccp4hnaalas {- at -} GMAIL {- dot -} COM
Date: 2008-10-01

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Subject: Anomalous correlation plot in scala
From: Daniele de Sanctis de {- dot -} sanctisd {- at -} GMAIL {- dot -} COM
Date: 2008-10-01

Subject: Re: precipitates
From: amit sharma 3112amit {- at -} GMAIL {- dot -} COM
Date: 2008-10-01

Dear All,
Many thanks for the suggestions.

Cheers,
Amit

2008/10/1 Chavas Leo

> Dear Amit --
> On 30 Sep 2008, at 14:16, amit sharma wrote:
>
> I am trying to crystallize a protein with a peptide attached to it via a
> 9aa linker. I am mostly getting precipitates in conditions carrying salts. I
> have tried optimization screens by varying the pH and salt concentrations,
> but in vain. Can anyone please suggest ways by which I could optimize the
> conditions further?
>
>
> Among all the possibilities, you can:
> - try to crystallize without salt ;
> - change the temperature ;
> - dilute your protein ;
> - add some isopropanol (or other) to make the solution "smoother" ;
> - change your linker size ;
> - ...
>
> HTH
> Kind regards.
>
> -- Leo --
> ------------------------------------------------------------
> Chavas Leonard, Ph.D. @ home
> Research Associate
> Marie Curie Actions Fellow
> ------------------------------------------------------------
> Faculty of Life Sciences
> The University of Manchester
> The Michael Smith Building
> Oxford Road
> Manchester Lancashire
> M13 9PT
> ------------------------------------------------------------
> Tel: +44(0)161-275-1586
> e-mail: Leonard.Chavas@manchester.ac.uk
> http://personalpages.manchester.ac.uk/staff/leonard.chavas/
>
>
>

--
Amit Sharma, Ph.D. Research Associate, Department of Biology,
University of York, YO10 5DD UK

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: precipitates
From: Chavas Leo ccp4hnaalas {- at -} GMAIL {- dot -} COM
Date: 2008-10-01

Next message:
Subject: Anomalous correlation plot in scala
From: Daniele de Sanctis de {- dot -} sanctisd {- at -} GMAIL {- dot -} COM
Date: 2008-10-01