[ccp4bb] Bin Number (0 -19) Put in Mtz file in Place of Rfree Flag -and- 5MC in DNA

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: resuspending precipitated protein
From: ANDY DODDS andy {- dot -} dodds6 {- at -} GOOGLEMAIL {- dot -} COM
Date: 2008-10-01

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Subject: Re: Bin Number (0 -19) Put in Mtz file in Place of Rfree Flag -and- 5MC in DNA
From: Kevin Cowtan cowtan {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2008-10-01

Subject: Bin Number (0 -19) Put in Mtz file in Place of Rfree Flag -and- 5MC in DNA
From: "John R {- dot -} Horton" jrhorto {- at -} EMORY {- dot -} EDU
Date: 2008-10-01

I've tried several ways to get an Rfree flag into my mtz
file...converting from a CNS to mtz (with existing flags) and
scalepack to mtz and etc etc...with the same result, i.e. that the
"bin number" that the reflection is in (0-19) is put in mtz file as
the Rfree flag and not 0 or 1. I was wondering if this has to do with
array size or something. This is a 1.42A dataset with 125982
reflections.

In addition, I am trying to get refmac to recognize a 5MC as a DNA
base with proper links in the backbone etc...what is the best way to
do this?
------------------------------------------------------------------------------------------------------------------
John R. Horton, Ph.D.
Assistant Professor (Research) E-
mail: jrhorto@emory.edu
Emory University School of Medicine Voice-
mail: 404-727-8492
Department of Biochemistry
FAX: 404-727-3746
Rollins Research Center, Suite G239
1510 Clifton Road
Atlanta, GA 30322
------------------------------------------------------------------------------------------------------------------

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: resuspending precipitated protein
From: ANDY DODDS andy {- dot -} dodds6 {- at -} GOOGLEMAIL {- dot -} COM
Date: 2008-10-01