[ccp4bb] R32 data

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: mtz library in windows
From: Alex XIE itn2000uk {- at -} GMAIL {- dot -} COM
Date: 2008-10-01

Next message:
Subject: Protein-DNA complex prepartion for crystallization
From: E rajakumar e_rajakumar {- at -} YAHOO {- dot -} COM
Date: 2008-10-01

Subject: R32 data
From: Josiah Obiero jmo012 {- at -} MAIL {- dot -} USASK {- dot -} CA
Date: 2008-10-01

Dear all,

I have set of data (~2.7A). After indexing, HKL2000 suggests a
rhombohedral space group or C centered monoclinic space group. I tried
molecular replacement with R32 ( 109.55 109.55 155.28 90.00 90.00
120.00) and C2 (121.092 109.315 81.537 90.000 97.874
90.000) (both have good merging statistics), but did not get any
solution. I also tried P1 ( 81.553 81.527 81.523 84.202
84.132 84.156) but did get any solution ( wt structure is known and
the only difference with the wt structure is that the new structure is
complexed with a smaller protein). I expect that there'll be some
domain movement so searched for individual domains in phaser.

The self rotation function in P1 showed both two fold and three fold
peaks but they were both off the centre. The data does not appear to be
twinned. I am curious to know if it is normal for cell dimensions to
drastically vary from one space group to another. I am running out of
ideas, could I be dealing with a wrong space group or pseudosymetry?

Any suggestions would be appreciated.

Thanks.

Josiah.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: mtz library in windows
From: Alex XIE itn2000uk {- at -} GMAIL {- dot -} COM
Date: 2008-10-01

Next message:
Subject: Protein-DNA complex prepartion for crystallization
From: E rajakumar e_rajakumar {- at -} YAHOO {- dot -} COM
Date: 2008-10-01