[ccp4bb] Protein-DNA complex prepartion for crystallization

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: R32 data
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Date: 2008-10-01

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Date: 2008-10-01

Subject: Protein-DNA complex prepartion for crystallization
From: E rajakumar e_rajakumar {- at -} YAHOO {- dot -} COM
Date: 2008-10-01

Dear All
Sorry for non-crystallography query. I am working on
DNA binding protein, while mixing DNA with protein for
preparing Protein-DNA complex for crystallization,
protein is precipitating. pI of the protein is 9.3
and in 15 mM HEPES 7.0, 150mM NaCl and 5% glycerol.
Concentration of the protein used for mixing with DNA
is 8 mg/mL. DNA to protein molar ratio is 1.2. Please
advise me how to prevent precipitation. Is changing of
buffer pH and adding divalent cation like MgCl2 can
help in preventing precipitation?
Thanking You
Rajakumara

E. Rajakumara
Postdoctoral Fellow
Strcutural Biology Program
Memorial Sloan-Kettering Cancer Center
New York-10021
NY
001 212 639 7986 (Lab)
001 917 674 6266 (Mobile)

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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: R32 data
From: Josiah Obiero jmo012 {- at -} MAIL {- dot -} USASK {- dot -} CA
Date: 2008-10-01

Next message:
Subject: Pittsburgh Diffraction Conference - deadline reminder
From: Clyde Smith csmith {- at -} SLAC {- dot -} STANFORD {- dot -} EDU
Date: 2008-10-01