Re: [ccp4bb] resuspending precipitated protein

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: resuspending precipitated protein
From: Juergen Bosch jbosch {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2008-10-01

6M Guanidiniumhydrochloride works pretty good, the question is only
will you be able to refold it correctly ?

I believe a better approach is to avoid precipitation in the first
place and optimize your purification procedure in such a way that it
works. When do you observe precipitate ? Dialysis, then us e a fast
desalting column instead.

Jürgen

On 1 Oct 2008, at 06:12, ANDY DODDS wrote:

> Hello,
>
> following on from a previous topic about precipitating protein, but I
> believe a distinct caveat of this warranting a separate thread, I
> would like to know people's experiences in trying to get precipitated
> protein back into solution? Is there a way or are there many ways?
>
> Any experiences would be welcome,
>
> yours,
>
> Andy

-
Jürgen Bosch
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
FAX: +1-206-685-7002
Web: http://faculty.washington.edu/jbosch

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Pittsburgh Diffraction Conference - deadline reminder
From: Clyde Smith csmith {- at -} SLAC {- dot -} STANFORD {- dot -} EDU
Date: 2008-10-01

Next message:
Subject: SOme wquestions about refining.......
From: AA awaan686 {- at -} STUDENT {- dot -} OTAGO {- dot -} AC {- dot -} NZ
Date: 2008-10-01