Re: [ccp4bb] Reading the old literature / truncate / refinement programs

- Protein crystallography

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: SUMMARY: losing zinc during crystallization
From: Sue Roberts suer {- at -} EMAIL {- dot -} ARIZONA {- dot -} EDU
Date: 2008-10-02

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Subject: Beamtime at SLS X06SA
From: Clemens Schulze-Briese clemens {- dot -} schulze {- at -} PSI {- dot -} CH
Date: 2008-10-03

Subject: Re: Reading the old literature / truncate / refinement programs
From: Pavel Afonine pafonine {- at -} LBL {- dot -} GOV
Date: 2008-10-03

Hi Pete,

just a remark:

On 9/25/2008 4:00 PM, Dunten, Pete W. wrote:
>
>
> I mentioned previously phenix.refine tosses your weak data if IMEAN,
> SIGIMEAN are chosen during refinement.
>

phenix.refine does not automatically remove the data based on sigma (it
does it by user's request only). phenix.refine removes only negative or
zero values for Iobs (Fobs).

> I'm wondering if this omission of weak Fobs from the Fobs-Fcalc
> difference map explains why the difference maps out of refmac seem to
> be more helpful in showing where to move atoms.
>

I would be interested to look into this and discuss some more if you
contact me directly with a particular example.

Cheers,
Pavel.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: SUMMARY: losing zinc during crystallization
From: Sue Roberts suer {- at -} EMAIL {- dot -} ARIZONA {- dot -} EDU
Date: 2008-10-02

Next message:
Subject: Beamtime at SLS X06SA
From: Clemens Schulze-Briese clemens {- dot -} schulze {- at -} PSI {- dot -} CH
Date: 2008-10-03