Re: [ccp4bb] Reading the old literature / truncate / refinement programs

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

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   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Beamtime at SLS X06SA
From: Clemens Schulze-Briese clemens {- dot -} schulze {- at -} PSI {- dot -} CH
Date: 2008-10-03

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From: Andreas_Förster docandreas {- at -} GMAIL {- dot -} COM
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Subject: Re: Reading the old literature / truncate / refinement programs
From: Frank von Delft frank {- dot -} vondelft {- at -} SGC {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2008-10-03

>>
>> I mentioned previously phenix.refine tosses your weak data if IMEAN,
>> SIGIMEAN are chosen during refinement.
>>
>
> phenix.refine does not automatically remove the data based on sigma
> (it does it by user's request only). phenix.refine removes only
> negative or zero values for Iobs (Fobs).
That is in fact the same as removing based on sigma: standard practice
has been for some time that no data is removed, ever.

At the refinement stage, that is. Of course, we do remove data at
merging, for various reasons which probably also need investigating
(e.g. "high res cutoff" = truncation; cf .Free Lunch).

phx.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Beamtime at SLS X06SA
From: Clemens Schulze-Briese clemens {- dot -} schulze {- at -} PSI {- dot -} CH
Date: 2008-10-03

Next message:
Subject: refinement of half molecules
From: Andreas_Förster docandreas {- at -} GMAIL {- dot -} COM
Date: 2008-10-03