[ccp4bb] Protein DNA complexes concentration "bis"

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Subject: Protein DNA complexes concentration "bis"
From: Chaix Denis denis {- dot -} chaix {- at -} CBS {- dot -} CNRS {- dot -} FR
Date: 2008-10-07

Dear all

For crystallisation assays and other experiments, I want to concentrate
2 protein-DNA complexes purified by gel filtration.

- The affinity of the protein for the DNA is 10 nM.
- I could concentrate the protein until 1.5 mg/ml (= 38 µM)
- I have already tried lyophylisation (before and after complex
creation) or amicon method without results (I can't manage to increase
the concentration).
- The complex could be a dimer or a tetramer on the DNA

Do you have any good idea about this?????

Thank you for your help and sorry for my first incomprehensible mail

CHAIX Denis

Centre de Biochimie Structurale
INSERM U554/CNRS 5048
29 rue de Navacelles
F-34090 Montpellier, France
Tel: 33 4 67 41 77 21
Fax: 33 4 67 41 79 13
e-mail:Denis.chaix@cbs.cnrs.fr
http://www.cbs.cnrs.fr

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Positions / CCP4 wiki
From: Kay Diederichs Kay {- dot -} Diederichs {- at -} UNI-KONSTANZ {- dot -} DE
Date: 2008-10-07

Next message:
Subject: Re: Positions / CCP4 wiki
From: Gerard DVD Kleywegt gerard {- at -} XRAY {- dot -} BMC {- dot -} UU {- dot -} SE
Date: 2008-10-07