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Re: [ccp4bb] Crystal growing in one direction...need suggestions |
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- Protein crystallographyMain steps:- Protein purification- Crystallisation Special:- Programs for crystallography- X-ray detectors Basic tutorials:- Chemistry- Protein - Peptide - Amino Acids Xtal community:- CCP4BB |
CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999Subject: Re: Crystal growing in one direction...need suggestions From: mesters mesters {- at -} BIOCHEM {- dot -} UNI-LUEBECK {- dot -} DE Date: 2008-10-14 Hi, you could also try microseed matrix screening in order to find new crystallization conditions. - Jeroen - shivesh kumar wrote: > Dear all, > I have crystallized a protein in MPD which is thin and growing in one > direction only. I have tried ethonal, DMSO, Proline, Glucose, Urea, > Sucrose, Detergent kit from Hampton and lot more alongwith temperature > variation. Need suggestions regarding improvement of xtal quality, > THICKNESS. > Thanx in advance. > Shivesh > -- Dr. Jeroen R. Mesters Gruppenleiter Strukturelle Neurobiologie und Kristallogenese Institut für Biochemie, Universität zu Lübeck Zentrum für Medizinische Struktur- und Zellbiologie Ratzeburger Allee 160, D-23538 Lübeck Tel: +49-451-5004065, Fax: +49-451-5004068 Http://www.biochem.uni-luebeck.de Http://www.iobcr.org Http://www.selfish-brain.org Http://www.opticryst.org -- If you can look into the seeds of time and say which grain will grow and which will not - speak then to me (Macbeth) -- CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999 |
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