Re: [ccp4bb] Semet in non-auxotrophic strains

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Semet in non-auxotrophic strains
From: Guenter Fritz guenter {- dot -} fritz {- at -} UNI-KONSTANZ {- dot -} DE
Date: 2008-11-03

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Subject: Re: Semet in non-auxotrophic strains
From: Cynthia Kinsland clk10 {- at -} CORNELL {- dot -} EDU
Date: 2008-11-03

Subject: Re: Semet in non-auxotrophic strains
From: Arthur Laganowsky art {- at -} MBI {- dot -} UCLA {- dot -} EDU
Date: 2008-11-03

Dear Jacob,

You can find several protocols for Se-Met labeling at:

http://www.doe-mbi.ucla.edu/local/protocols

Good Luck,
Art

> Dear Crystallographers,
>
> I have been using an E coli strain called Tuner (DE3) pLacI, which is not
> auxotrophic for methionine, nor is there an auxotrophic analogue
> available.
> It may be that my protein can be expressed in other strains, but this one
> works well right now, and it is usually better to stay with what works
> well.
> Has anybody tried growing semet protein in non-auxotrophic strains, or are
> there tricks to using non-auxotrophs for semet protein?
>
> Thanks,
>
> Jacob
>
> *******************************************
> Jacob Pearson Keller
> Northwestern University
> Medical Scientist Training Program
> Dallos Laboratory
> F. Searle 1-240
> 2240 Campus Drive
> Evanston IL 60208
> lab: 847.491.2438
> cel: 773.608.9185
> email: j-keller2@northwestern.edu
> *******************************************
>

Arthur Laganowsky, M.S.
David Eisenberg Laboratory
University of California, Los Angeles
Chemistry and Biochemistry Graduate Student
611 Charles E. Young Drive East
219 Boyer
Los Angeles, California 90095
USA

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Semet in non-auxotrophic strains
From: Guenter Fritz guenter {- dot -} fritz {- at -} UNI-KONSTANZ {- dot -} DE
Date: 2008-11-03

Next message:
Subject: Re: Semet in non-auxotrophic strains
From: Cynthia Kinsland clk10 {- at -} CORNELL {- dot -} EDU
Date: 2008-11-03