[ccp4bb] phased MR

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: ESRF School - 2nd - 5th February 2009
From: nurizzo nurizzo {- at -} ESRF {- dot -} FR
Date: 2008-11-04

Next message:
Subject: Re: phased MR
From: Pietro Roversi pietro {- dot -} roversi {- at -} PATH {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2008-11-04

Subject: phased MR
From: Thomas Edwards T {- dot -} A {- dot -} Edwards {- at -} LEEDS {- dot -} AC {- dot -} UK
Date: 2008-11-04

Dear BB,

I would like to ask for some advice on phased molecular replacement if
possible.

I have a MR model that has so far not proved successful with Phaser,
Molrep, Amore, Beast etc.

I have SeMet MAD data to 3.6A that gives decent looking anomalous
difference peaks, looks stable in mlphare, and produces solvent
flattened maps to 2.8A in DM that look like the density might be a
sensible shape - wrt solvent gaps etc - but not interpretable so far (I
will be trying phasing in SHARP, CNS etc).

In the mean time, is there a good way to combine phases that may be
slightly sensible with molecular replacement? Things may also be
complicated by the possibility of NCS translations...

Any advice gratefully received.

Many thanks to all those who continue to provide excellent suggestions,

Cheers

Ed

______________
T.Edwards Ph.D.
Garstang 8.53d
Astbury Centre for Structural Molecular Biology
University of Leeds, Leeds, LS2 9JT
Telephone: 0113 343 3031

http://www.bmb.leeds.ac.uk/staff/tae/
If you're not part of the solution, you're part of the precipitate.
~Henry J. Tillman

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: ESRF School - 2nd - 5th February 2009
From: nurizzo nurizzo {- at -} ESRF {- dot -} FR
Date: 2008-11-04

Next message:
Subject: Re: phased MR
From: Pietro Roversi pietro {- dot -} roversi {- at -} PATH {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2008-11-04