Re: [ccp4bb] phased MR

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

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   - Protein
   - Peptide
   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: phased MR
From: Thomas Edwards T {- dot -} A {- dot -} Edwards {- at -} LEEDS {- dot -} AC {- dot -} UK
Date: 2008-11-04

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From: pramod madoori madooripramod {- at -} GMAIL {- dot -} COM
Date: 2008-11-04

Subject: Re: phased MR
From: Pietro Roversi pietro {- dot -} roversi {- at -} PATH {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2008-11-04

Dear Ed,
in the past we have successfully searched in an
electron density map (computed in the whole cell) with Molrep.

If you have a second crystal form and you can cut the density of the
monomer off, Phaser also gives very good results when
searching with that electron density - after that your maps can be
improved with dmmulti cross-crystal averaging.

Good luck!

Pietro

--
Pietro Roversi
EP Abraham Fellow in Biochemistry - Lincoln College - Oxford
Sir William Dunn School of Pathology, Oxford University
South Parks Road, Oxford OX1 3RE, England UK
Tel. 0044-1865-275385

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: phased MR
From: Thomas Edwards T {- dot -} A {- dot -} Edwards {- at -} LEEDS {- dot -} AC {- dot -} UK
Date: 2008-11-04

Next message:
Subject: pymol cartoon problem
From: pramod madoori madooripramod {- at -} GMAIL {- dot -} COM
Date: 2008-11-04