Re: [ccp4bb] phased MR

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Postdoc position
From: Lucy Malinina lucy {- dot -} malinina {- at -} GMAIL {- dot -} COM
Date: 2008-11-04

Next message:
Subject: is it possible or is it existing ?
From: Jayashankar s {- dot -} jayashankar {- at -} GMAIL {- dot -} COM
Date: 2008-11-04

Subject: Re: phased MR
From: Anastassis Perrakis a {- dot -} perrakis {- at -} NKI {- dot -} NL
Date: 2008-11-04

Hi -

I would not use mlphare for anything marginal (to be honest not at all).

Both SHARP (use eternal pdb file in top page of the gui) and the new
Phaser (look at the doc for scripts for this case) can do what you want.

Tassos

On Nov 4, 2008, at 11:55, Thomas Edwards wrote:

> Dear BB,
>
> I would like to ask for some advice on phased molecular replacement
> if possible.
>
> I have a MR model that has so far not proved successful with Phaser,
> Molrep, Amore, Beast etc.
>
> I have SeMet MAD data to 3.6A that gives decent looking anomalous
> difference peaks, looks stable in mlphare, and produces solvent
> flattened maps to 2.8A in DM that look like the density might be a
> sensible shape - wrt solvent gaps etc - but not interpretable so far
> (I will be trying phasing in SHARP, CNS etc).
> In the mean time, is there a good way to combine phases that may be
> slightly sensible with molecular replacement? Things may also be
> complicated by the possibility of NCS translations…
>
> Any advice gratefully received.
>
> Many thanks to all those who continue to provide excellent
> suggestions,
> Cheers
> Ed
>
> ______________
> T.Edwards Ph.D.
> Garstang 8.53d
> Astbury Centre for Structural Molecular Biology
> University of Leeds, Leeds, LS2 9JT
> Telephone: 0113 343 3031
> http://www.bmb.leeds.ac.uk/staff/tae/
> If you're not part of the solution, you're part of the precipitate.
> ~Henry J. Tillman
>
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Postdoc position
From: Lucy Malinina lucy {- dot -} malinina {- at -} GMAIL {- dot -} COM
Date: 2008-11-04

Next message:
Subject: is it possible or is it existing ?
From: Jayashankar s {- dot -} jayashankar {- at -} GMAIL {- dot -} COM
Date: 2008-11-04