Re: [ccp4bb] phased MR

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: m-3m vs. m-3
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2008-11-04

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From: Sebastiano Pasqualato sebastiano {- dot -} pasqualato {- at -} IFOM-IEO-CAMPUS {- dot -} IT
Date: 2008-11-04

Subject: Re: phased MR
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2008-11-04

You can read phases into both MOLREP and Amore and search for the
translation against the phased map.. It often gives good results.

Eleanor

Pietro Roversi wrote:
> Dear Ed,
> in the past we have successfully searched in an
> electron density map (computed in the whole cell) with Molrep.
>
> If you have a second crystal form and you can cut the density of the
> monomer off, Phaser also gives very good results when
> searching with that electron density - after that your maps can be
> improved with dmmulti cross-crystal averaging.
>
> Good luck!
>
> Pietro
>
>

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: m-3m vs. m-3
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2008-11-04

Next message:
Subject: tricoordinated ion?
From: Sebastiano Pasqualato sebastiano {- dot -} pasqualato {- at -} IFOM-IEO-CAMPUS {- dot -} IT
Date: 2008-11-04